PCRWhat is PCR?
- PCR is a method in which we amplify our DNA sample in to multiplies copies.
- Component which require for PCR.
- Nucleotide
- Primer
- DNA polymerase
- Nucleotide
- In PCR 4 nucleotide are present these are Guanine,Cytocine,Adenine,Thymine
- DNA Polymerase.
- Dna polymerase is a enzyme which digest the double stranded DNA in to single stranded DNA temperature require for DNA is 120C.
- Primer
- In double stranded stand separate and primer to both side of DNA
- Procedure
- There are following steps for Pcr.
- Initial Denaturation
- In first step DNA is denature 2 strand to single strand at 94 degree centigrade for 5 minutes
- In final step DNA completely single strand.
- Anealing
- Primer are attached on complementary stranded at this temperature is low down at 56 degree centigrade for 2 minutes.
- Extension
- In this step temperature again increase 72 degree centigrade and strand are extended.
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